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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">biosel</journal-id><journal-title-group><journal-title xml:lang="ru">Биотехнология и селекция растений</journal-title><trans-title-group xml:lang="en"><trans-title>Plant Biotechnology and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2658-6266</issn><issn pub-type="epub">2658-6258</issn><publisher><publisher-name>VIR</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2658-6266-2023-3-o4</article-id><article-id custom-type="elpub" pub-id-type="custom">biosel-195</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИЗУЧЕНИЕ ГЕНЕТИЧЕСКИХ РЕСУРСОВ РАСТЕНИЙ С ИСПОЛЬЗОВАНИЕМ МЕТОДОВ МОЛЕКУЛЯРНОЙ ГЕНЕТИКИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>STUDY OF PLANT GENETIC RESOURCES USING MOLECULAR GENETICS METHODS</subject></subj-group></article-categories><title-group><article-title>Изучение линий мягкой пшеницы, полученных с участием синтетической формы Авродес, в отношении их устойчивости к жёлтой ржавчине</article-title><trans-title-group xml:lang="en"><trans-title>A study of bread wheat lines from crosses with the synthetic form Avrodes in regard to their yellow rust resistance</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0007-2786-617X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Давоян</surname><given-names>Э. Р.</given-names></name><name name-style="western" xml:lang="en"><surname>Davoyan</surname><given-names>E. R.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Эдвард Румикович Давоян, доктор биологических наук, заведующий, отдел биотехнологии, Национальный центр зерна им. П.П. Лукьяненко</p><p>350012 Россия, Краснодар, Центральная Усадьба КНИИСХ</p></bio><bio xml:lang="en"><p>Edvard R. Davoyan, Dr. Sci. (Biology), Head, Department of Biotechnology</p><p>Krasnodar, Russia 350012</p></bio><email xlink:type="simple">davayan@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0001-9389-6234</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бебякина</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Bebyakina</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ирина Викторовна Бебякина, кандидат биологических наук, ведущий научный сотрудник, отдел биотехнологии, Национальный центр зерна им. П.П. Лукьяненко</p><p>350012 Россия, Краснодар, Центральная Усадьба КНИИСХ</p></bio><bio xml:lang="en"><p>Irina V. Bebyakina, Cand. Sci. (Biology), Leading Researcher, Department of Biotechnology</p><p>Krasnodar,  350012 Russia</p></bio><email xlink:type="simple">irina.bebyakina@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9495-2026</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Давоян</surname><given-names>Р. О.</given-names></name><name name-style="western" xml:lang="en"><surname>Davoyan</surname><given-names>R. O.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Румик Оганесович Давоян, доктор биологических наук, главный научный сотрудник, отдел биотехнологии, Национальный центр зерна им. П.П. Лукьяненко</p><p>350012 Россия, Краснодар, Центральная Усадьба КНИИСХ</p></bio><bio xml:lang="en"><p>Rumik O. Davoyan, Dr. Sci. (Biology), Chief Researcher, Department of Biotechnology</p><p>Krasnodar,  350012 Russia</p></bio><email xlink:type="simple">davoyanro@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0005-5656-7174</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Болдаков</surname><given-names>Д. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Boldakov</surname><given-names>D. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Дмитрий Максимович Болдаков, научный сотрудник, отдел биотехнологии, Национальный центр зерна им. П.П. Лукьяненко</p><p>350012 Россия, Краснодар, Центральная Усадьба КНИИСХ</p></bio><bio xml:lang="en"><p>Dmitry M. Boldakov, Researcher, Department of Biotechnology</p><p>Krasnodar, 350012  Russia</p></bio><email xlink:type="simple">boldasar@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7101-9639</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бадаева</surname><given-names>Е. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Badaeva</surname><given-names>E. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Екатерина Дмитриевна Бадаева, доктор биологических наук, ведущий научный сотрудник, лаборатория генетических основ идентификации растений, отдел генетики растений, Институт общей генетики им. Н.И. Вавилова РАН</p><p>119991 Россия, ГСП-1, Москва, ул. Губкина, 3</p></bio><bio xml:lang="en"><p>Ekaterina D. Badaeva, Dr. Sci. (Biology), Leading Researcher, Laboratory of Genetic Basis of Plant Identification, Department of Plant Genetics</p><p>3, Gubkina Street, Moscow, GSP-1,  119991 Russia</p></bio><email xlink:type="simple">katerinabadaeva@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8460-6119</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Адонина</surname><given-names>И. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Adonina</surname><given-names>I. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ирина Григорьевна Адонина, кандидат биологических наук, научный сотрудник, лаборатория молекулярной генетики и цитогенетики растений, ИЦиГ СО РАН</p><p>630090 Россия, Новосибирск, пр. Академика Лаврентьева, 10 </p></bio><bio xml:lang="en"><p>Irina G. Adonina, Cand. Sci. (Biology), Researcher, Laboratory of Plant Molecular Genetics and Cytogenetics</p><p>10, Akademika Lavrentieva Avenue, Novosibirsk,   630090 Russia</p></bio><email xlink:type="simple">adonina@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8590-847X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Салина</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Salina</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Елена Артемовна Салина, доктор биологических наук, отдел молекулярной генетики растений, ИЦиГ СО РАН</p><p>630090 Россия, Новосибирск, пр. Академика.Лаврентьева,10</p><p> </p></bio><bio xml:lang="en"><p>Elena A. Salina, Dr. Sci. (Biology), Chief Researcher, Department of Molecular Genetics of Plants</p><p>10, Academician Lavrentjev Avenue, Novosibirsk, 630090 Russia</p></bio><email xlink:type="simple">salina@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0001-4899-5671</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зинченко</surname><given-names>А. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Zinchenko</surname><given-names>A. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Александра Николаевна Зинченко, научный сотрудник, отдел биотехнологии, Национальный центр зерна им. П.П. Лукьяненко</p><p>350012 Россия, Краснодар, Центральная Усадьба КНИИСХ</p></bio><bio xml:lang="en"><p>Alexandra N. Zinchenko, Researcher, Department of Biotechnology</p><p>Krasnodar,  350012 Russia</p></bio><email xlink:type="simple">zinchenk0an@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0001-7615-006X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зубанова</surname><given-names>Ю. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Zubanova</surname><given-names>Yu. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Юлия Сергеевна Зубанова, старший научный сотрудник, отдел биотехнологии, Национальный центр зерна им. П.П. Лукьяненко</p><p>350012 Россия, Краснодар, Центральная Усадьба КНИИСХ</p></bio><bio xml:lang="en"><p>Julia S. Zubanova, Senior Researcher, Department of Biotechnology</p><p>Krasnodar,  350012 Russia</p></bio><email xlink:type="simple">zubanova-yu@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Национальный центр зерна им. П.П. Лукьяненко<country>Россия</country></aff><aff xml:lang="en">National Center of Grain named after P.P. Lukyanenko<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Институт общей генетики им. Н.И. Вавилова Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Institute of General Genetics of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>14</day><month>01</month><year>2024</year></pub-date><volume>6</volume><issue>3</issue><fpage>25</fpage><lpage>34</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Давоян Э.Р., Бебякина И.В., Давоян Р.О., Болдаков Д.М., Бадаева Е.Д., Адонина И.Г., Салина Е.А., Зинченко А.Н., Зубанова Ю.С., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Давоян Э.Р., Бебякина И.В., Давоян Р.О., Болдаков Д.М., Бадаева Е.Д., Адонина И.Г., Салина Е.А., Зинченко А.Н., Зубанова Ю.С.</copyright-holder><copyright-holder xml:lang="en">Davoyan E.R., Bebyakina I.V., Davoyan R.O., Boldakov D.M., Badaeva E.D., Adonina I.G., Salina E.A., Zinchenko A.N., Zubanova Y.S.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://biosel.elpub.ru/jour/article/view/195">https://biosel.elpub.ru/jour/article/view/195</self-uri><abstract><p>Для передачи мягкой пшенице устойчивости к жёлтой ржавчине (Puccinia striiformis f. sp. tritici Eriks.) от Aegilops speltoides Tausch, (2n = 14) использовалась синтетическая геномно-замещенная форма Авродес (AABBSS). Проведено изучение 24 интрогрессивных линий мягкой пшеницы, полученных с применением формы Авродес. Отобраны устойчивые к жёлтой ржавчине линии P07-L.02, P07-L.1, P07-L.17, P07-L.43, P07-L.19, АS12-88, АS12-06, АS12-07, АS12-51, Asp81-21, Asp63-21, Asp053-21, Asp04-21, Asp022-19, Asp023-19 и Asp029-20, которые могут быть использованы в качестве новых доноров устойчивости к болезни. С помощью дифференциального окрашивания хромосом (С-окраска) и флуоресцентной in situ гибридизации (FISH) у линий идентифицирован генетический материал Ae. speltoides, переданный в форме замещения хромосомы 5S(5D) и транслокаций T5BS.5BL-5SL, T2DL.2DS-2SS, T5D, а также транслокация T1BL.1RS от Secale cereale L. В ходе работы выявлено, что линии с единичными транслокациями T1BL.1RS и T5BS.5BL-5SL были восприимчивы к жёлтой ржавчине, тогда как линии, у которых идентифицированы транслокация T2DL.2DS-2SS и замещения 5S(5D), а также линии с транслокациями T1BL.1RS, T2DL.2DS-2SS и T5D проявляли устойчивость к болезни. Предположительно, отобранные нами интрогрессивные линии, полученные с участием Авродес, могут нести новые гены или локусы устойчивости к жёлтой ржавчине.</p></abstract><trans-abstract xml:lang="en"><p>The genome-substituted synthetic form Avrodes (AABBSS) was used for transferring resistance to yellow rust (Puccinia striiformis f. sp. tritici Eriks.) from Aegilops speltoides Tausch, (2n = 14) to bread wheat. The study involved 24 introgressive lines of bread wheat obtained using the Avrodes form. Yellow rust resistant lines P07-L.02, P07-L.1, P07-L.17, P07-L.43, P07-L.19, AS12-88, AS12-06, AS12-07, AS12- 51, Asp81-21, Asp63-21, Asp053-21, Asp04-21, Asp022-19, Asp023-19 and Asp029-20 were selected and can be used as new donors of disease resistance. The use of differential chromosome staining (C-banding) and fluorescence in situ hybridization (FISH) identified the genetic material of Ae. speltoides transmitted in the form of 5S(5D) chromosome substitution and translocations of T5BS.5BL-5SL, T2DL.2DS-2SS, T5D, as well as translocation of T1BL.1RS from Secale cereale L. The work revealed that the lines with single translocations of T1BL.1RS and T5BS.5BL-5SL were susceptible to yellow rust, while the lines in which the T2DL.2DS-2SS translocation and 5S(5D) substitutions were identified, as well as the lines with translocations of T1BL.1RS, T2DL.2DS-2SS and T5D showed resistance to the disease. Presumably, the selected introgression lines, obtained by means of crosses with Avrodes, may carry new genes or loci for yellow rust resistance.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>T. aestivum</kwd><kwd>Ae. speltoides</kwd><kwd>Авродес</kwd><kwd>интрогрессивные линии</kwd><kwd>устойчивость к жёлтой ржавчине</kwd></kwd-group><kwd-group xml:lang="en"><kwd>T. aestivum</kwd><kwd>Ae. speltoides</kwd><kwd>Avrodes</kwd><kwd>introgressive lines</kwd><kwd>resistance to yellow rust</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Генотипирование гибридного материала мягкой пшеницы методом флуоресцентной in &lt;i&gt;situ&lt;/i&gt;  гибридизации (FISH) выполнено при поддержке проекта Министерства науки и высшего образования FWNR-2022-0017.</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>Genotyping of bread wheat hybrid material using fluorescence &lt;i&gt;in situ&lt;/i&gt;  hybridization (FISH) was supported by the Ministry of Science and Higher Education project FWNR-2022-0017</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Adonina I.G., Petrash N.V., Timonova E.M., Khristov Y.A., Salina E.A. 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