bioselБиотехнология и селекция растенийPlant Biotechnology and Breeding2658-62662658-6258VIR10.30901/2658-6266-2024-3-o3biosel-223Research ArticleКРАТКИЕ СООБЩЕНИЯBRIEF COMMUNICATIONSМолекулярно-генетический инструментарий для анализа дифференциальной экспрессии генов субъединиц β-конглицининов соиA molecular genetic toolkit for the differential expression analysis of soybean β-conglycinin subunit geneshttps://orcid.org/0009-0008-3104-1696КатрушенкоА. А.KatrushenkoA. A.

Анастасия Андреевна Катрушенко, лаборант-исследователь, лаборатория биотехнологии, ФНЦ ВНИИ сои

Россия 675000, Амурская область, Благовещенск, Игнатьевское шоссе, 19

Anastasia A. Katrushenko, Research Assistant, Laboratory of Biotechnology, FRC ARSRIS

19, Ignat'evskoe Highway, Blagoveshchensk, Amur Region, 675000 Russia

aak@vniisoi.ruhttps://orcid.org/0000-0001-6655-1049ТимкинП. Д.TimkinP. D.

Павел Дмитриевич Тимкин, младший научный сотрудник, лаборатория биотехнологии, ФНЦ ВНИИ сои

Россия 675000, Амурская область, Благовещенск, Игнатьевское шоссе, 19

Pavel D. Timkin, Junior Researcher, Laboratory of Biotechnology, FRC ARSRIS 

19, Ignat'evskoe Highway, Blagoveshchensk, Amur Region, 675000 Russia

tpd@vniisoi.ruhttps://orcid.org/0000-0002-8578-9818ПензинА. А.PenzinA. A.

Андрей Андреевич Пензин, научный сотрудник, лаборатория биотехнологии, ФНЦ ВНИИ сои

Россия 675000, Амурская область, Благовещенск, Игнатьевское шоссе, 19

Andrey A. Penzin, Researcher, Laboratory of Biotechnology, FRC ARSRIS 

19, Ignat'evskoe Highway, Blagoveshchensk, Amur Region, 675000 Russia

paa@vniisoi.ruФедеральный научный центр «Всероссийский научно-исследовательский институт сои»РоссияFederal Scientific Center «All-Russian Research Institute of Soybean»Russian Federation202429092024735360Copyright © Катрушенко А.А., Тимкин П.Д., Пензин А.А., 20242024Катрушенко А.А., Тимкин П.Д., Пензин А.А.Katrushenko A.A., Timkin P.D., Penzin A.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://biosel.elpub.ru/jour/article/view/223

Большая часть белков в семенах сои являются запасными, среди которых β-конглицинины и глицинины, необходимы растению для прорастания семян. В то же время, они являются наиболее ценными соевыми белками, использующимися в пищевой промышленности, так как их субъединичный состав и доля от общего белка может влиять на качество получаемого пищевого продукта. β-конглицинины являются тримерами, состоящими из разного состава субъединиц, обозначаемых как α', α, β и кодируемых генами CG-1, CG-3 и CG-4 соответственно. Для ПЦР-анализа использовали модельный сорт сои ‘Сентябринка’. В качестве матрицы использовали комплементарную ДНК, синтезированную на основе РНК, выделенной из семян исследуемого сорта. Применяли пары праймеров к транскриптам генов CG-1, CG-3 и CG-4, созданные in silico. В результате ПЦР и анализа полученных электрофореграмм подобраны оптимальные температуры отжига праймеров для генов CG-1, CG-3 и CG-4, при которых проявляется только характерный фрагмент. Таким образом, для комплексного изучения качественного и количественного состава соевого белка создан молекулярно-генетический инструментарий, с помощью которого можно осуществлять дальнейший анализ дифференциальной экспрессии генов, отвечающих за синтез субъединиц β-конглицининов сои.

The majority of proteins in soybean seeds are storage ones, including β-conglycinin and glycinin, which are necessary for seed germination. At the same time, they are the most valuable soy proteins used in the food industry, since their subunit composition and proportion of total protein can affect the quality of the resulting food product. β-conglycinins are trimers with different composition of subunits which are designated as α', α, β and encoded by the CG-1, CG-3, and CG-4 genes, respectively. The PCR analysis employed a model soybean cultivar ‘Sentyabrinka’. A complementary DNA synthesized from the RNA isolated from seeds of the studied cultivar served as a template. The in silico created pairs of primers for CG-1, CG-3, and CG-4 gene transcripts were used. As the result of PCR and the analysis of the obtained electrophoregrams, optimal annealing temperatures of primers for the CG-1, CG-3 and CG-4 genes were selected, at which only the characteristic fragment was observed. Thus, a molecular genetic toolkit has been developed for a comprehensive study of the qualitative and quantitative composition of soybean protein and can be used for further analysis of differential expression of genes responsible for the synthesis of β-conglycinin subunits.

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The authors declare that there are no conflicts of interest present.