bioselБиотехнология и селекция растенийPlant Biotechnology and Breeding2658-62662658-6258VIR10.30901/2658-6266-2019-4-o3biosel-60Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕORIGINAL ARTICLEРазработка хромосомо-специфичных ДНК-маркеров для изучения интрогрессивной гибридизации картофеля с диким мексиканским аллотетраплоидным видом Solanum stoloniferum Schltdl.Development of chromosome-specific markers for a study on introgressive hybridization of potato with the wild Mexican allotetraploid species Solanum stoloniferum Schltdlhttps://orcid.org/0000-0001-8334-8069АнтоноваО. Ю.AntonovaO. Yu.https://orcid.org/0000-0002-3106-4926ЕрмишинА. П.YermishinA. P.ermishin@igc.byЛевыйА. В.LevyA. V.АгееваА. С.AgeevaA. S.https://orcid.org/0000-0001-9747-8622ВоронковаЕ. В.VoronkovaE. V.https://orcid.org/0000-0002-2605-6569ГавриленкоТ. А.GavrilenkoT. A.tatjana9972@yandex.ruФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н. И. ВавиловаРоссияN.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationИнститут генетики и цитологии НАН БеларусиБеларусьIInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusBelarus201921042020242435Copyright © Антонова О.Ю., Ермишин А.П., Левый А.В., Агеева А.С., Воронкова Е.В., Гавриленко Т.А., 20192019Антонова О.Ю., Ермишин А.П., Левый А.В., Агеева А.С., Воронкова Е.В., Гавриленко Т.А.Antonova O.Y., Yermishin A.P., Levy A.V., Ageeva A.S., Voronkova E.V., Gavrilenko T.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://biosel.elpub.ru/jour/article/view/60

Для вовлечения в селекцию дикого аллотетраплоидного мексиканского вида картофеля Solanum stoloniferum Schltdl. (геномный состав ААВВ) обычно используют пентаплоидные межвидовые гибриды (ААААВ) с культурным картофелем S. tuberosum L. (АААА), которые в дальнейшем включают в программу возвратных скрещиваний. Поскольку у таких гибридов в мейозе ожидается синапсис гомологичных хромосом А генома, возникает вопрос о перспективах интрогрессивной гибридизации генетического материала суб-генома В S. stoloniferum. В связи с этим актуальна разработка различных схем селекционного процесса, позволяющих повысить вероятность интрогрессии генетического материала суб-генома B в геном культурного картофеля. В предыдущих исследованиях были разработаны четыре схемы вовлечения S. stoloniferum в селекционный процесс, которые включают возвратные скрещивания с культурным картофелем различных межвидовых гибридов: гексаплоидного (геномный состав ААААВВ, традиционная схема интрогрессии), тетраплоидного гибрида (предполагаемый геномный состав АААВ) и потомства, полученного от его самоопыления, а также пентаплоидного межвидового гибрида (предполагаемый геномный состав АААВВ). В настоящей работе представлены первые результаты исследований по разработке хромосомоспецифичных маркеров для идентификации у межвидовых гибридов генетического материала S. stoloniferum. В скрещиваниях был использован перспективный образец S. stoloniferum PI 205522, высокоустойчивый к фитофторозу и Y-вирусу картофеля, у которого выявлен ряд ДНК маркеров генов устойчивости к этим патогенам. Для изучения особенностей интрогрессии генетического материала S. stoloniferum был создан набор из 23 SSR- и CAPS-маркеров с известной хромосомной локализацией в геноме А S. tuberosum, выявляющих полиморфизм родительских генотипов - диплоидного клона IGC 10/1.21 культурного картофеля S. tuberosum и образца PI 205522 S. stoloniferum. Все маркеры, специфичные для родительского образца дикого вида, были выявлены как у триплоидного (ААВ), так и у пентаплоидного (АААВВ) гибридов S. stoloniferum × S. tuberosum. Созданный набор маркеров будет использован для оценки эффективности различных схем интрогрессии генетического материала S. stoloniferum, в которых получены гибриды второго и третьего поколений беккроссов межвидовых гибридов с культурным картофелем.

In order to involve valuable germplasm of the wild Mexican allotetraploid potato species Solanum stoloniferum Schltdl. (genomic composition ААВВ) into breeding, pentaploid interspecific hybrids (ААAAВ) with cultivated potato S. tuberosum L. (АААА) and their backcross progenies are usually used. Homologous synapsis in meiosis of such hybrids is expected only between chromosomes of the A subgenome, therefore a question arose about a possibility of introgressing genetic material of the subgenome B into the A genome of cultivated potato. In this connection, development of various schemes for the B subgenome introgression into the genome of cultivated potato is considered as a topical issue. The previous research has yielded four schemes of S. stoloniferum involvement into breeding, which imply backcrossing with cultivated potato of the following interspecific hybrids: (1) hexaploids (genomic composition ААААВВ, the conventional introgression scheme), (2) tetraploids (putatively, АААВ), (3) self-pollination progeny of a 4x hybrid and (4) pentaploid hybrids with a putative genome composition of АААВВ. The present paper presents the first results of the development of chromosome-specific DNA markers for the identification of S. stoloniferum chromosomes in interspecific hybrids. An S. stoloniferum accession PI 205522 with a high degree of resistance to late blight and PVY had been found to possess several DNA-markers of the R-genes conferring resistance to these pathogens and was used in hybridization as a promising parent. A set of 23 SSR- and CAPS markers with the known chromosome location in S. tuberosum was generated. These markers detect polymorphism between parent genotypes, i.e., the diploid clone IGC 10/1.21 of cultivated potatoes S. tuberosum, and accession PI 205522 of S. stoloniferum. All the markers specific for the wild species were found in triploid (ААВ) and pentaploid (АААВВ) hybrids of S. stoloniferum × S. tuberosum. This set of markers will be used for efficiency assessment of different schemes for S. stoloniferum genetic material introgression into the obtained BC2-BC3 generations after crossing the interspecific hybrids with cultivated potato.

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The authors declare that there are no conflicts of interest present.