bioselБиотехнология и селекция растенийPlant Biotechnology and Breeding2658-62662658-6258VIR10.30901/2658-6266-2020-2-o4biosel-70Research ArticleОБЗОРЫREVIEW ARTICLESИспользование методов молекулярной цитогенетики в исследованиях отдаленных гибридов картофеляThe use of molecular cytogenetic methods in the investigation of distant potato hybridshttps://orcid.org/0000-0002-0104-9418МакароваТ. О.MakarovaТ.  О.

190000 г. Санкт-Петербург, ул. Б. Морская, 42, 44

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000

tamaramakarova333@gmail.comФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н. И. Вавилова (ВИР)N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)202029122020323038Copyright © Макарова Т.О., 20202020Макарова Т.О.Makarova Т.О.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://biosel.elpub.ru/jour/article/view/70

Данный обзор посвящен рассмотрению результатов изучения межвидовой гибридизации, полиплоидии и филогенетических отношений видов рода Solanum и представителей близких к нему таксонов, с использованием методов молекулярной цитогенетики, таких как гибридизация ДНК-ДНК in situ: геномная – GISH и флуоресцентная – FISH. Метод GISH был использован для определения геномного состава и происхождения ряда диких видов секции Petota. Метод FISH используют для выявления коллинеарности геномов разных видов. Такого рода исследования позволяют проводить сравнительный анализ кариотипов и геномов, а также способствуют лучшему пониманию взаимодействия хромосом в мейозе у гибридов. В данном обзоре сделан акцент на изучении диких аллополиплоидных видов картофеля с помощью методов молекулярной цитогенетики, рассмотрены результаты анализа искусственно созданных межродовых и межвидовых гибридов рода Solanum и их потомства.

This paper reviews the results of studies of interspecies hybridization, polyploidization, as well as phylogenetic relationships of Solanum species and members of closely related taxa by such molecular cytogenetic techniques as genomic (GISH) and fluorescent (FISH) DNA-DNA in situ hybridization. The latter was used to determine the genomic composition and origin of wild species of the Petota section, while the FISH technique was used for detecting intergenomic collinearity. The combination of these two types of research made possible a comparative analysis of karyotypes and genomes, thus allowing a better understanding of the meiotic interchromosomal interactions in hybrids. This review primarily focuses on the studies of wild allopolyploid potato species and artificially created intergeneric and interspecific hybrids of the genus Solanum and their offspring.

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The authors declare that there are no conflicts of interest present.