bioselБиотехнология и селекция растенийPlant Biotechnology and Breeding2658-62662658-6258VIR10.30901/2658-6266-2020-1-o5biosel-74Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL ARTICLEСоздание линии табака со сниженными антифидантными свойствами по отношению к колорадскому жукуCreating a tobacco line with a weaker antifeedant property against colorado potato beetleКостинаН. Е.KostinaN. E.

630090 г. Новосибирск

Novosibirsk 630090

СпасельниковаА. В.SpaselnikovaA. V.

630090 г. Новосибирск

Novosibirsk 630090

ЕгороваА. А.EgorovaA. A.

630090 г. Новосибирск

Novosibirsk 630090

КолосовскаяЕ. В.KolosovskayaE. V.

630090 г. Новосибирск

Novosibirsk 630090

ДомрачевД. В.DomrachevD. V.

630090 г. Новосибирск

Novosibirsk 630090

РомановаА. В.RomanovaA. V.

630090 г. Новосибирск

Novosibirsk 630090

ТуманянС. Р.TumanyanS. R.

630039 г. Новосибирск

Novosibirsk 630039

ХамасС.ChamasS.

Gatersleben 06466

КумленЙ.KumlehnJ.

Gatersleben 06466

ДубовскийИ. М.DubovskiyI. M.

630039 г. Новосибирск630501 Новосибирская область, р.п. Краснообск

Novosibirsk 630039Krasnoobsk Sett., Novosibirsk Province 630501

https://orcid.org/0000-0001-8626-1831ГерасимоваС. В.GerasimovaS. V.

630090 г. Новосибирск

Novosibirsk 630090

gerson@bionet.nsc.ruИнститут цитологии и генетики СО РАНРоссияInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут органической химии СО РАНРоссияInstitute of Organic Chemistry of Siberian Branch of the Russian Academy of SciencesRussian FederationНовосибирский государственный аграрный университетРоссияNovosibirsk State Agrarian UniversityRussian FederationLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)GermanyНовосибирский государственный аграрный университет; Сибирский федеральный научный центр агробиотехнологий РАНРоссияNovosibirsk State Agrarian University; Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of SciencesRussian Federation202010082020312430Copyright © Костина Н.Е., Спасельникова А.В., Егорова А.А., Колосовская Е.В., Домрачев Д.В., Романова А.В., Туманян С.Р., Хамас С., Кумлен Й., Дубовский И.М., Герасимова С.В., 20202020Костина Н.Е., Спасельникова А.В., Егорова А.А., Колосовская Е.В., Домрачев Д.В., Романова А.В., Туманян С.Р., Хамас С., Кумлен Й., Дубовский И.М., Герасимова С.В.Kostina N.E., Spaselnikova A.V., Egorova A.A., Kolosovskaya E.V., Domrachev D.V., Romanova A.V., Tumanyan S.R., Chamas S., Kumlehn J., Dubovskiy I.M., Gerasimova S.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://biosel.elpub.ru/jour/article/view/74никотинNicotiana tabacumLeptinotarsa decemlineataтоксичностьсемейство генов BBLCas9нРНКnicotineNicotiana tabacumLeptinotarsa decemlineatatoxicityBBL gene familyCas9gRNAПолучение модифицированных линий табака проводили при поддержке гранта Российского фонда фундаментальных исследований (проект № 18-416-543004), исследование на колорадском жуке выполнено за счет гранта Российского научного фонда (проект №19-1600019).Generation of modified tobacco lines was supported by a grant from the Russian Foundation for Basic Research (Project No. 18-416-543004), and the Colorado potato beetle research was supported by a grant from the Russian Science Foundation (Project No. 19-16-00019).ReferencesAlyokhin A., Baker M., Mota-Sanchez D., Dively G., Grafius E. Colorado Potato Beetle Resistance to Insecticides. American Journal of Potato Research. 2008;85:395413. DOI: 10.1007/s12230-008-9052-0.Alyokhin A., Baker M., Mota-Sanchez D., Dively G., Grafius E. Colorado Potato Beetle Resistance to Insecticides. American Journal of Potato Research. 2008;85:395413. DOI: 10.1007/s12230-008-9052-0.Chen P.-Y., Wang C.-K., Soong S.-C., To K.-Y. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Molecular Breeding. 2003;11:287-293. DOI: 10.1023/A:1023475710642Chen P.-Y., Wang C.-K., Soong S.-C., To K.-Y. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Molecular Breeding. 2003;11:287-293. DOI: 10.1023/A:1023475710642Домрачев, Д.В., Егорова, А.А., Колошина, К.А., Герасимова, С.В. Постановка методики анализа содержания алкалоидов в тканях некоторых видов семейства пасленовых методом высокоэффективной жидкостной хроматографии. В кн.: Объединенное научное мероприятие "День поля" в рамках ФАНО России "Развитие селекции и семеноводства картофеля" и научная конференция "Теоретические основы и прикладные исследования в селекции и семеноводстве картофеля": тезисы докладов, г. Новосибирск, Россия, 1-5 августа 2018 г. Новосибирск; 2018. С.15). DOI: 10.18699/Potato-2018-11Domrachev D.V., Egorova A.A., Koloshina K.A., Gerasimova S.V. High-performance liquid chromatography method for the evaluation of alkaloids in Solanaceae. (Postanovka metodiki analiza soderzhaniya alkaloidov v tkanyakh nekotorykh vidov semeystva paslenovykh metodom vysokoeffektivnoy zhidkostnoy khromatografii.). In: Joint scientific event "Field Day" within the framework of the FANO of Russia "Development of Potato Breeding and Seed Production" and the scientific conference "Theoretical Foundations and Applied Research in Potato Breeding and Seed Production" (Ob"yedinennoye nauchnoye meropriyatiye "Den' polya" v ramkakh FANO Rossii "Razvitiye selektsii i semenovodstva kartofelya" i nauchnaya konferentsiya "Teoreticheskiye osnovy i prikladnyye issledovaniya v selektsii i semenovodstve kartofelya"): abstracts; 2018 August 1-5; Novosibirsk, Russia. Novosibirsk; 2018. p.15 [In Russian] DOI: 10.18699/Potato-2018-11Fauser F., Schiml S., Puchta H. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant Journal. 2014;79:348-359. DOI: 10.1111/tpj.12554Fauser F., Schiml S., Puchta H. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant Journal. 2014;79:348-359. DOI: 10.1111/tpj.12554Hsiao T.H., Fraenkel G. The Role of Secondary Plant Substances in the Food Specificity of the Colorado Potato Beetle. Annals of the Entomological Society of America. 1968;61(2):485-493. DOI: 10.1093/aesa/61.2.485Hsiao T.H., Fraenkel G. The Role of Secondary Plant Substances in the Food Specificity of the Colorado Potato Beetle. Annals of the Entomological Society of America. 1968;61(2):485-493. DOI: 10.1093/aesa/61.2.485Иванова К.А., Спаселикова А.В., Шумный В.К., Герасимова С.В. Генымишени для метаболической инженерии представителей семейства Solanaceae: эволюция и структурная организация. Биотехнология и селекция растений. 2018;1(1):34-42.). DOI: 10.30901/2658-6266-2018-1-34-42Ivanova K.A., Spaselikova A.V., Shumny V.K., Gerasimova S.V. The target genes for Solanaceae secondary metabolism engineering: evolution and genome organization. Plant Biotechnology and Breeding. 2018;1(1):34-42. [In Russian] DOI: 10.30901/2658-6266-2018-1-34-42Kajikawa M., Shoji T., Kato A., Hashimoto T. Vacuole-Localized Berberine Bridge Enzyme-Like Proteins Are Required for a Late Step of Nicotine Biosynthesis in Tobacco. Plant Physiology. 2011;155:2010-2022. DOI: 10.1104/pp.110.170878.Kajikawa M., Shoji T., Kato A., Hashimoto T. Vacuole-Localized Berberine Bridge Enzyme-Like Proteins Are Required for a Late Step of Nicotine Biosynthesis in Tobacco. Plant Physiology. 2011;155:2010-2022. DOI: 10.1104/pp.110.170878.Lewis R.S., Drake-Stowe K.E., Heim C., Steede T., Smith W., Dewey R.E. Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes. Frontiers in Plant Science. 2020;11. DOI: 10.3389/fpls.2020.00368Lewis R.S., Drake-Stowe K.E., Heim C., Steede T., Smith W., Dewey R.E. Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes. Frontiers in Plant Science. 2020;11. DOI: 10.3389/fpls.2020.00368Murashige T., Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 1962;15:473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.xMurashige T., Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 1962;15:473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.xSchachtsiek J., Stehle F. Nicotine-free, nontransgenic tobacco (Nicotiana tabacum L.) edited by CRISPR/Cas9. Plant Biotechnology Journal. 2019;17:2228-2230. DOI: 10.1111/pbi.13193Schachtsiek J., Stehle F. Nicotine-free, nontransgenic tobacco (Nicotiana tabacum L.) edited by CRISPR/Cas9. Plant Biotechnology Journal. 2019;17:2228-2230. DOI: 10.1111/pbi.13193Sierro N., Battey J.N.D., Ouadi S., Bakaher N., Bovet L., Willig A., et al. The tobacco genome sequence and its comparison with those of tomato and potato. Nature Communications. 2014;5:3833. DOI: 10.1038/ncomms4833Sierro N., Battey J.N.D., Ouadi S., Bakaher N., Bovet L., Willig A., et al. The tobacco genome sequence and its comparison with those of tomato and potato. Nature Communications. 2014;5:3833. DOI: 10.1038/ncomms4833Weber D. Colorado beetle: pest on the move. Pesticide Outlook. 2003;14(6):256-259. DOI: 10.1039/b314847pWeber D. Colorado beetle: pest on the move. Pesticide Outlook. 2003;14(6):256-259. DOI: 10.1039/b314847p

The authors declare that there are no conflicts of interest present.