bioselБиотехнология и селекция растенийPlant Biotechnology and Breeding2658-62662658-6258VIR10.30901/2658-6266-2018-1-52-63biosel-8Research ArticleОБЗОРЫREVIEW ARTICLESCryoconservation methods for vegetatively propagated cropsУхатоваЮ. В.UkhatovaY. V.

190000, г. Санкт-Петербург, ул. Б. Морская, д. 42, 44

42-44, Bolshaya Morskaya St., St. Petersburg, 190000

ГавриленкоТ. А.GavrilenkoT. A.

190000, г. Санкт-Петербург, ул. Б. Морская, д. 42, 44;

199004, г. Санкт-Петербург, Университетская набережная, д. 7/9.

42-44, Bolshaya Morskaya St., St. Petersburg, 190000;

7/9, Universitetsrfya naberezhnaya, St. Petersburg, 199034.

 

tatjana9972@yandex.ruФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н. И. Вавилова (ВИР)РоссияN. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н. И. Вавилова (ВИР); Санкт-Петербургский государственный университетРоссияN. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); St. Petersburg State UniversityRussian Federation201805022019115263Copyright © Ухатова Ю.В., Гавриленко Т.А., 20192019Ухатова Ю.В., Гавриленко Т.А.Ukhatova Y.V., Gavrilenko T.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://biosel.elpub.ru/jour/article/view/8

This article presents a review of cryopreservation methods used to create cryocollections of plant genetic resources. The application of cryopreservation methods for the long-term conservation of the vegetatively propagated crops started relatively recently. About 60 years ago, the first methods of programmable (slow) freezing of plant objects were developed. The modern methods include such fast freezing techniques as encapsulation-dehydration, vitrification, encapsulation-vitrification, droplet-method, and droplet-vitrification. These methods are used for cryopreservation of accessions from the field genbanks and samples from in vitro collections. The review considers the main factors determining the viability and regeneration rates of explants after freezing-rewarming. The greatest impact on the efficiency of post-cryogenic explants recovery is provided by such factors as the method of the initial microplants pre-treatment, explant and cryoprotectant type, duration of the explant treatment with cryoprotectants, the composition of the nutrient medium for the post-cryogenic recovery, and the accession genotypic characteristics. The review discusses the rules for the collections formation and cryobanking development. The data on the largest cryocollections of cultivated plant species are presented.

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The authors declare that there are no conflicts of interest present.