New approaches to registration and conservation of domestic cultivars of berry crops in the VIR Genebank on the example of red raspberry and black currant

A collection of nomenclatural standards is being created at VIR for domestic cultivars of various crops in accordance with the International Code of Nomenclature for Cultivated Plants (ICNCP). A new complex strategy was proposed for vegetatively propagated crops for registering domestic cultivars received from their authors in the VIR genebank. In addition to the creation of nomenclature standards, the strategy includes the development of a genetic passport of a cultivar and the use of biotechnological methods to preserve explants (buds, meristems) isolated from plant material transferred by breeders to the VIR Herbarium. This approach can be used for any vegetatively propagated crop applying a protocol developed specifically for an individual crop. For raspberry and black currant varieties, the collecting of plant material, its preparation for the registration of nomenclature standards and the preservation of viable samples under controlled in vitro conditions have specific features. This article provides detailed protocols for performing the mentioned work for raspberry and black currant varieties. In addition, the article summarizes the first results of the implementation of our proposed strategy on the example of domestic raspberry and black currant varieties created in various breeding centers of Russia. Three years of joint work of VIR researchers and breeders from four breeding centers in five regions of the country have resulted in creation of nomenclature standards for 20 raspberry varieties, as well as for five black currant varieties bred at VIR. Samples of thirteen raspbery cultivars and samples of four black currant cultivars, genetically identical to nomenclature standards, were introduced into in vitro culture; four raspberry cultivars have been placed in the VIR cryobank for the long-term cryopreservation.

1. Antonova O.Yu., Klimenko N.S., Rybakov D.A., Fomina N.A., Zheltova V.V., Novikova L.Yu., Gavrilenko T.A. SSR analysis of modern Russian potato varieties using DNA samples of nomenclatural standards. Plant Biotechnology and Breeding. 2020;3(4):77-96. DOI: 10.30901/2658-6266-2020-4-o2

2. Bagmet L.V., Chebotok E.M., Shlyavas A.V. Nomenclatural standards of black currant cultivars bred by Sverdlovsk Horticultural Breeding Station. Part I. Agricultural Science Euro-North-East. 2021;22(6):873-886. DOI: 10.30766/2072-9081.2021.22.6.873-886

3. Bagmet L.V., Chebotok E.M., Shlyavas A.V. Nomenclatural standards of black currant cultivars bred by Sverdlovsk Horticultural Breeding Station. Part I. Agricultural Science Euro-North-East. 2022;23(1):69-80. DOI: 10.30766/2072-9081.2022.23.1.69-80

4. Belozor N.I. Herbarization of cultivated plants (Guidelines) (Gerbarizatsiya kulturnykh rastenii: (Metodicheskie ukazaniya). Leningrad: VIR; 1989.

5. Brickell C.D., Alexander C., Cubey J.J., David J.C., Hoffman M.H.A., Leslie A.C., Malécot V., Xiaobai J. (eds). International code of nomenclature for cultivated plants. Ed. 9. Scripta Horticulturae. 2016;18:I-XVII+1-190.

6. Gavrilenko T.A., Chukhina I.G. Nomenclatural standards of modern Russian potato cultivars preserved at the VIR herbarium (WIR): A new approach to cultivar genepool registration in a genebank. Plant Biotechnology and Breeding. 2020;3(3):6-17. DOI: 10.30901/2658-6266-2020-3-o2

7. Dunaeva S.E., Pendinen G.I., Antonova O.Y., Shvachko N.A., Ukhatova Y.V., Shuvalova L.E. Volkova N.N., Gavrilenko T.A. Preservation of vegetatively propagated crops in in vitro and cryo collections: methodological guidelines. (Sokhraneniye vegetativno razmnozhayemykh kultur v in vitro i krio kollektsiyakh: metodicheskiye ukazaniya). T.A. Gavrilenko (ed.). 2nd ed. St. Petersburg: VIR; 2017.

8. International Code of Nomenclature for Cultivated Plants. Division III–VI, Appendix I–IX. Chukhina I.G., Miftakhova S.R., Dorofeуev V.I. (transl.). Translation of: “International Code of Nomenclature for Cultivated Plants. Ed. 9. Scripta Horticulturae. 2016;18:I-XVII+1-190ˮ. Vavilovia. 2022;5(1):41-70. DOI: 10.30901/2658-3860-2022-1-41-70

9. Kamnev A.M., Yagovtseva N.D., Dunaeva S.E., Gavrilenko T.A., Chukhina I.G. Nomenclatural standards of raspberry cultivars bred in the Altai. Vavilovia. 2021;4(2):26-43. DOI: 10.30901/2658-3860-2021-2-26-43

10. Kamnev A.M., Dunaeva S.E., Nevostrueva E.Yu., Kuzmina A.A., Gavrilenko T.A., Chukhina I.G. Nomenclatural standards of raspberry varieties bred by the Sverdlovsk breeding station of gardening and Novosibirsk Zonal Gardening Station. Vavilovia. [in press] 2022.

11. Khlestkina E.K. Genetic resources in Russia: from collections to bioresource centers. Proceedings on Applied Botany, Genetics and Breeding. 2022;183(1):9-30. DOI: 10.30901/2227-8834-2022-1-9-30

12. Klimenko N.S., Gavrilenko T.А., Chukhina I.G., Gadzhiev N.M., Evdokimova Z.Z., Lebedeva V.А. Nomenclatural standards and genetic passports of potato cultivars bred in the Leningrad Scientific Research Institute of Agriculture “Belogorka”. Plant Biotechnology and Breeding. 2020;3(3):18-54. DOI: 10.30901/2658-6266-2020-3-o3

13. Rybakov D.А., Аntonova O.Yu., Chukhina I.G., Fomina N.А., Klimenko N.S., Zheltova V.V., Meleshin А.А., Kochieva E.Z., Oves E.V., Аpshev Kh.Kh., Simakov E.А., Gavrilenko T.А. Nomenclatural standards and genetic passports of potato cultivars bred in the A.G. Lorkh All-Russian Potato Research Institute of Potato Farming. Plant Biotechnology and Breeding. 2020;3(4):5-52. DOI: 10.30901/2658-6266-2020-4-o1

14. Sedov E.N. (ed.). Pomology. V. 5: Strawberry, raspberry, nut and rare crops. Orel: VNIISPB Publisher; 2014.

15. Sedov E.N. Integrated research programms for selection fruit and berry crops and their effectiveness. Breeding and cultivar cultivation of horticultural crops = Selektsiia i sortorazvedenie sadovykh kultur. 2016;3(1):126-129.

16. State Register for Selection Achievements Admitted for Usage (National List). Vol. 1 “Plant varieties” (official publication) / Ministry of Agriculture of Russia; 2021.

17. Tikhonova O.A., Shabliuk N.O., Gavrilenko T.A., Dunaeva S.E., Talovina G.V. Nomenclatural standards of black currant cultivars bred at VIR. Vavilovia. 2021;4(2):3-25. DOI: 10.30901/2658-3860-2021-2-3-25

18. Turland N.J., Wiersema J.H., Barrie F.R., Greuter W., Hawksworth D.L., Herendeen P.S., Knapp S., Kusber W.-H., Li D.-Z., Marhold K., May T.W., McNeill J., Monro A.M., Prado J., Price M.J., Smith G.F. (eds.). International Code of Nomenclature for algae, fungi, and plants (Shenzhen Code). Adopted by the Nineteenth International Botanical Congress Shenzhen, China, July 2017. Regnum Vegetabile 159. Glashütten: Koeltz Botanical Books; 2018. DOI: 10.12705/Code.2018

19. Ukhatova Y.V., Dunaeva S.E., Antonova O.Y., Apalikova O.V., Pozdniakova K.S., Novikova L.Y., Shuvalova L.E., Gavrilenko T.A. Cryopreservation of red raspberry cultivars from the VIR in vitro collection using a modified droplet vitrification method. In Vitro Cellular and Developmental Biology – Plant. 2017;53:394-401. DOI: 10.1007/s11627-017-9860-3

20. Yevdokimenko S.N., Podgaetsky M.A. State of raspberry assortment in Russia and problems of its improvement. Pomiculture and small fruits culture in Russia. 2019;59:294-300.