A molecular genetic toolkit for the differential expression analysis of soybean β-conglycinin subunit genes
https://doi.org/10.30901/2658-6266-2024-3-o3
Abstract
The majority of proteins in soybean seeds are storage ones, including β-conglycinin and glycinin, which are necessary for seed germination. At the same time, they are the most valuable soy proteins used in the food industry, since their subunit composition and proportion of total protein can affect the quality of the resulting food product. β-conglycinins are trimers with different composition of subunits which are designated as α', α, β and encoded by the CG-1, CG-3, and CG-4 genes, respectively. The PCR analysis employed a model soybean cultivar ‘Sentyabrinka’. A complementary DNA synthesized from the RNA isolated from seeds of the studied cultivar served as a template. The in silico created pairs of primers for CG-1, CG-3, and CG-4 gene transcripts were used. As the result of PCR and the analysis of the obtained electrophoregrams, optimal annealing temperatures of primers for the CG-1, CG-3 and CG-4 genes were selected, at which only the characteristic fragment was observed. Thus, a molecular genetic toolkit has been developed for a comprehensive study of the qualitative and quantitative composition of soybean protein and can be used for further analysis of differential expression of genes responsible for the synthesis of β-conglycinin subunits.
Supporting agencies: The work was carried out within the framework of the Federal project "Agrarian science – a step into the future development of the agro-industrial complex" on topic No. 082-3-2023-0007
About the Authors
A. A. Katrushenko
Federal Scientific Center «All-Russian Research Institute of Soybean»
Russian Federation
Russian Federation
Anastasia A. Katrushenko, Research Assistant, Laboratory of Biotechnology, FRC ARSRIS
19, Ignat'evskoe Highway, Blagoveshchensk, Amur Region, 675000 Russia
P. D. Timkin
Federal Scientific Center «All-Russian Research Institute of Soybean»
Russian Federation
Russian Federation
Pavel D. Timkin, Junior Researcher, Laboratory of Biotechnology, FRC ARSRIS
19, Ignat'evskoe Highway, Blagoveshchensk, Amur Region, 675000 Russia
A. A. Penzin
Federal Scientific Center «All-Russian Research Institute of Soybean»
Russian Federation
Russian Federation
Andrey A. Penzin, Researcher, Laboratory of Biotechnology, FRC ARSRIS
19, Ignat'evskoe Highway, Blagoveshchensk, Amur Region, 675000 Russia
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For citations:
Katrushenko A.A., Timkin P.D., Penzin A.A. A molecular genetic toolkit for the differential expression analysis of soybean β-conglycinin subunit genes. Plant Biotechnology and Breeding. 2024;7(3):53-60. (In Russ.) https://doi.org/10.30901/2658-6266-2024-3-o3
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