Germination of soybean seeds after low-temperature storage and its dependence on weather and climate conditions in the places of reproduction of accessions

Background. Soybean (Glycine max (L.) Merr.) is a microbiotic whose seeds quickly lose germination in uncontrolled conditions at room temperatures. This determines the need to preserve accessions in special conditions. Low temperature storage (LTS) allows to maintain high seed germination ability, but not for all accessions. Therefore, it is relevant to assess the results of LTS at the VIR Genebank and to make an attempt to determine the best geographical conditions for growing plants and harvesting seeds that will be subjected to LTS. Materials and methods. The work was carried out on 312 soybean accessions from the VIR collection, originating from almost the entire area of soybean cultivation. The accessions used in the research were reproduced at three VIR experiment stations (ES), namely Adler ES, Kuban ES (Krasnodar Territory) and the Far East ES (Primorsky Territory). The seeds were harvested in 1999 to 2017 and stored in sealed foil laminated bags at –10°C from 2002 to 2021. The accessions were removed from LTS and germination assessed in 2022. Results. At the beginning of LTS, seed germination (G_i) ranged from 12 to 100% (averaging 79.1%), while that after LTS (G_r) ranged from 1 to 97% (57.8% on average). The retention of germination ability was expressed as the germination index (GI = G_r / G_i), which ranged from 0.02 to 1.73 (an average of 0.72). All the indicators of germination in the range of up to 20 years of LTS were not associated with the duration of LTS and with the number of years before LTS (0.5-4 years in our research). The climatic conditions characteristic of the place of reproduction had a significant impact on G_i and G_r, but not on GI. The average G_i of seeds obtained at the Adler ES and Kuban ES did not differ significantly (77.4% and 75.7%), while that of seeds harvested at the Far East ES was significantly higher (84.0%). G_r of the accessions from the Far East ES (64.3%) was also greater than that of accessions from the Kuban ES (52.2%) and Adler ES (57.2%). The excessively high sum of temperatures above 10°C (recorded in some years at all stations) reduced G_i, G_r but had little effect on GI. The highest values of G_i, G_r, and GI were demonstrated by the accessions maturing in 101-120 days. Additionally drying of the most late-ripening accessions (later than 140 days) in sheaves under an awning yielded seeds with high germination ability and good LTS tolerance. Conclusion. The seeds from different places of reproduction of accessions differed in germination before and after LTS, and, on an average, the highest values were demonstrated by the seeds harvested at the Far East ES, where the temperature regime is closer to the optimal one for soybeans. The seed germination index showed relative independence from the conditions of reproduction of accessions, including the climate and weather conditions, as well as the initial germination ability and LTS duration.

1. Arif M.A.R., Tripodi P., Waheed M.Q., Afzal I., Pistrick S., Schütze G., Börner A. Genetic analyses of seed longevity in Capsicum annuum L. in cold storage conditions. Plants. 2023;12:1321. DOI: 10.3390/plants12061321

2. Ballesteros D., Walters C. Solid-state biology and seed longevity: a mechanical analysis of glasses in pea and soybean embryonic axes. Frontiers in Plant Science. 2019;10:920. DOI: 10.3389/fpls.2019.00920

3. Chebrolu K.K., Fritschi F.B., Ye S., Krishnan H.B., Smith J.R., Gillman J. D. Impact of heat stress during seed development on soybean seed metabolome. Metabolomics. 2016;12:28. DOI: 10.1007/s11306-015-0941-1

4. De Vitis M., Hay F.R., Dickie J.B., Trivedi C., Choi J., Fiegener R. Seed storage: maintaining seed viability and vigor for restoration use. Restoration Ecology. 2020;28(S3):249-255. DOI: 10.1111/rec.13174

5. Desheva G., Petrova S., Deshev M. Germinability of soybean seeds stored more than 30 years in the Bulgarian national seed genebank. World Scientific News. 2017;69:29-46.

6. Ellis R.H. Temporal patterns of seed quality development, decline, and timing of maximum quality during seed development and maturation. Seed Science Research. 2019;29:135-142. DOI: 10.1017/ S0960258519000102

7. Genebank standards for plant genetic resources for food and agriculture. Rome: FAO; 2014. Available from: https://www.fao.org/4/i3704e/i3704e.pdf [accessed Jun. 21, 2024]

8. Genebank Standards. 1994. Food and Agriculture Organization of the United Nations. Rome: International Plant Genetic Resources Institute; 1994. Available from: https://cropgenebank.sgrp.cgiar.org/images/file/learning_space/genebank_standards.pdf [accessed June 21, 2024].

9. GOST 12038-84. Agricultural seeds. Methods for determination of germination. Moscow: Standartinform; 2011. [in Russian]

10. GOST 12041-82. Seed of farm crops. Method for determination of moisture content. Moscow: Standartinform; 2011. [in Russian]

11. Kalemba E.M., Corbineau F., Kumar S.P.J. Editorial: Molecular basis of seed longevity. Frontiers in Plant Science. 2023;14:1138139. DOI: 10.3389/fpls.2023.1138139

12. Kandil A.A., Sharief A.E., Sheteiwy M.S. Effect of seed storage periods, conditions and materials on germination of some soybean seed cultivars. American Journal of Experimental Agriculture. 2013;3(4):1020-1043. DOI: 10.9734/AJEA/2013/3590

13. Karim M.A., Utsunomiya N., Shigenaga S. Effect of sodium chloride on germination and growth of hexaploid triticale at early seedling stage. Japanese Journal of Crop Science. 1992;61:279-284. DOI: 10.1626/jcs.61.279

14. Kiran Babu P., Radhamani J., Aravind J., Varghese E., Tyagi R.K. Field performance of 30-year-old soybean germplasm conserved in Indian National Genebank. Plant Genetic Resources. 2018;31(2):152-163. DOI: 10.5958/0976-1926.2018.00018.9

15. Nagel M., Kranner I., Neumann K., Rolletschek H., Seal C.E., Colville L., Fernández-Marín B., Börner A. Genome-wide association mapping and biochemical markers reveal that seed ageing and longevity are intricately affected by genetic background and developmental and environmental conditions in barley. Plant, Cell & Environment. 2015;38(6):1011-1022. DOI: 10.1111/pce.12474

16. Nakagawa A.C.S., Ario N., Tomita Y., Tanaka S., Murayama N., Mizuta C., Iwaya-Inoue M., Ishibashi Y. High temperature during soybean seed development differentially alters lipid and protein metabolism. Plant Production Science, 2020;23(4):504-512. DOI: 10.1080/1343943X.2020.1742581

17. Novikova L.Y., Bulakh P.P., Nekrasov A.Y., Seferova I.V. Soybean response to weather and climate conditions in the Krasnodar and Primorye territories of Russia over the past decades. Agronomy. 2020;10(9):1278. DOI: 10.3390/agronomy10091278

18. Pritchard H.W., Dickie J.B. Predicting seed longevity: the use and abuse of seed viability equations. In book: Seed Conservation: Turning Science into Practice. UK: Royal Botanic Gardens Kew; 2003.

19. Rao P.J.M., Pallavi M., Bharathi Y., Priya P.B., Sujatha P., Prabhavathi K. Insights into mechanisms of seed longevity in soybean: a review. Frontiers in Plant Science. 2023;14:1206318. DOI: 10.3389/fpls.2023.1206318

20. Renard J., Niñoles R., Martínez-Almonacid I., Gayubas B., Mateos-Fernández R., Bissoli G., Bueso E., Serrano R., Gadea J. Identification of novel seed longevity genes related to oxidative stress and seed coat by genome-wide association studies and reverse genetics. Plant, Cell & Environment. 2020;43:2523-2539. DOI: 10.1111/pce.13822

21. Safina G.F., Filipenko G.I. Longevity of seeds at storage and its predicting by the accelerated ageing method. Proceedings on Applied Botany, Genetics and Breeding. 2013;174:124-131. [in Russian]

22. Silaeva O.I. Storage of seeds collections of the world's plant resources in conditions low positive temperatures – assessment, status, prospects. Proceedings on Applied Botany, Genetics and Breeding. 2012;169:230-239. [in Russian]

23. Solberg S.O., Yndgaard F., Andreasen Ch., von Bothmer R., Loskutov I.G., Asdal A. Long-term storage and longevity of orthodox seeds: a systematic review. Frontiers in Plant Science. 2020;11:1007. DOI: 10.3389/fpls.2020.01007

24. Stepanov V.N. Biological classification of agricultural plants of field culture. Izvestiya of Timiryazev Agricultural Academy. 1957;(2):5-29. [in Russian]

25. Storozheva N.N. Impact of long-term storage of crop seeds in permafrost soil strata on viability and phenotypic variability (Vliyaniye dlitel'nogo khraneniya semyan sel'skokhozyaystvennykh kul'tur v usloviyakh tolshchi mnogoletnemerzlykh gruntov na zhiznesposobnost' i fenotipicheskuyu izmenchivost) [dissertation]. Yakutsk; 2006. [in Russian]

26. Tekrony D.M., Egli D.B., Phillips A.D. Effect of field weathering on the viability and vigor of soybean seed. Agronomy Journal. 1980;72(5):749-753. DOI: 10.2134/agronj1980.00021962007200050014x

27. Teplykh A.A., Prokhorova E.V. The influence of spruce seeds longevity on their practical germinating ability. Vestnik of Volga State University of Technology. Series «Forest. Ecology. Nature management». 2018;2(38):19-28. [in Russian]. DOI: 10.15350/2306-2827.2018.2.19

28. Vavilov N.I. Botanical and geographical principles of breeding (The doctrine of source material in breeding) (Botaniko-geograficheskiye osnovy selektsii (Ucheniye ob iskhodnom materiale v selektsii)). In: Academician N.I. Vavilov. Selected Works (Akademik N.I. Vavilov. Izbrannye trudy). Moscow; Leningrad: Nauka; 1960. Vol. 2. p.21-70. [in Russian]

29. Yamasaki F., Domon E., Tomooka N., Baba-Kasai A., Nemoto H., Ebana K. Thirty-year monitoring and statistical analysis of 50 species' germinability in genebank medium-term storage suggest specific characteristics in seed longevity. Seed Science and Technology. 2020;48(2):269-287. DOI: 10.15258/sst.2020.48.2.14