A new marker for the detection of A-type chloroplast DNA in long-term stored herbarium specimens of cultivated potato species
https://doi.org/10.30901/2658-6266-2025-3-o1
Abstract
The Herbarium of cultivated plants of the world, their wild relatives and weeds (WIR) at the N.I. Vavilov All-Russian institute of plant genetic resources (VIR) houses Russia's largest collection of historical herbarium specimens of cultivated potato species. The plant samples were collected in 1925-1928 by S.V. Yuzepchuk and S.M. Bukasov, members of an expedition to Latin American countries organized by N.I. Vavilov. This material is of undoubted interest for molecular genetics research. However, there are methodological limitations due to varying degrees of DNA degradation in plants preserved long-term in herbarium collections. The original set of PCR markers specific to different loci of plastid DNA, developed by Japanese researchers K. Hosaka and R. Sanetomo (2012), is widely used for studying the genetic diversity and origin of cultivated potato species maintained in field and in vitro collections of various Genebanks. Applying this marker set in full for detecting different types of chloroplast DNA (cpDNA) in historical herbarium specimens is problematic, as some primers in this set generate amplicons larger than 1000 bp. In this study, new primers A494 were developed to detect A-type plastid DNA in ~100-year-old herbarium specimens of cultivated potato species. The efficiency of A494 primers, with the use of which amplicons of the expected size were obtained for 22 of 25 herbarium specimens, was significantly higher compared to the results obtained with primers A from the Hosaka and Sanetomo (2012) set, that is, amplicons for five out of 25 specimens. The expanded marker set for detecting various cpDNA types will be used in further studies of historical herbarium collection of cultivated potato preserved in the VIR Herbarium.
Supporting agencies: The research was carried out according to the State Assignment to VIR, Topics FGEM-2022-0006 and FGEM-2022-0008
About the Authors
N. A. Oskina
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Natalia A. Oskina, Researcher, Department of Biotechnology, VIR
42, 44, Bolshaya Morskaya Street, St. Petersburg, 190000 Russia
O. Yu. Antonova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Olga Yu. Antonova, Cand. Sci. (Biology), Leading Researcher, Head, Laboratory of Molecular Breeding and DNA Profiling, Department of Biotechnology, VIR
42, 44, Bolshaya Morskaya Street, St. Petersburg, 190000 Russia
T. A. Gavrilenko
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Tatjana A. Gavrilenko, Dr. Sci. (Biology), Chief Researcher, Department of Biotechnology, VIR
42, 44, Bolshaya Morskaya Street, St. Petersburg, 190000 Russia
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For citations:
Oskina N.A., Antonova O.Yu., Gavrilenko T.A. A new marker for the detection of A-type chloroplast DNA in long-term stored herbarium specimens of cultivated potato species. Plant Biotechnology and Breeding. 2025;8(3):43-54. (In Russ.) https://doi.org/10.30901/2658-6266-2025-3-o1
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