Особенности организации дуплицированных копий ортологов генов AtTT2 и AtTT8 арабидопсиса в геноме аллотетраплоидного хлопчатника

Arabidopsis thaliana L. является классическим модельным объектом, у которого подробно описаны механизмы регуляции биосинтеза флавоноидных пигментов с участием генов AtTT2, относящихся к семейству R2R3-MYB, и AtTT8 из семейства bHLH-MYC. Актуальным остается вопрос о судьбе дуплицированных копий у аллополиплоидных видов, возникших в результате межвидовой гибридизации, удобной моделью для изучения которого является хлопчатник вида Gossypium hirsutum L. В субгеномах At и Dt G. hirsutum идентифицированы копии генов GhTT2, GhMYB10 и GhTT8, являющиеся ортологами соответствующих генов A. thaliana. В настоящей работе на основе секвенирования аллелей указанных регуляторных генов у образцов хлопчатника c контрастной окраской волокна и гипокотиля оценивается роль аллополиплоидизации в эволюции дуплицированных регуляторных генов, кодирующих компоненты MBW-комплекса: транскрипционные факторы семейств R2R3-MYB, bHLH-MYC, WD40. Для генов семейства R2R3-MYB хлопчатника выявлен асимметричный характер эволюции, при котором гены субгенома Аt эволюционируют быстрее и находятся под менее строгим очищающим отбором по сравнению с гомеологами субгенома Dt. Секвенированные последовательности впервые депонированы в международную базу данных GenBank (NCBI).

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