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Variability of economically important traits of hemp depending on the region of cultivation

https://doi.org/10.30901/2658-6266-2025-4-o8

Abstract

Increasing the area under a given agricultural crop largely depends on the adaptive potential of new cultivars or those in production. The objective of this study was to comparatively assess the variability of economically important traits in hemp cultivars of the Central Russian ecotype ‘Nadezhda’, ‘Lyudmila’, ‘Surskaya’, and ‘Vera’ in the Middle Volga region and the western part of the Central Non-Chernozem Zone of Russia. In 2023-2024, the research was conducted in experimental fields at the Federal Scientific Center for Bast Crops in the Penza and Smolensk regions. The agroclimatic conditions of the regions varied significantly in terms of moisture availability. The sum of active temperatures in the Penza Region was 2397°C in 2023 and 2234°C in 2024, with precipitation of 177 mm and 156 mm, respectively. In the Smolensk Region, the sum of active temperatures was 2299°C in 2023, and 2385°C in 2024, with precipitation of 236 mm and 358 mm, respectively. The favorable climatic conditions during the two years of testing allowed for a significant realization of the cultivars’ potential, as observed under the conditions of the originator. In the Smolensk Region, maximum plant height reached 191 cm, technical length 162 cm, and seed weight per plant was 7.8 g. In the Penza Region, these values reached 281 cm in height, 262 cm in technical length, and 18.7 g in seed weight per plant. Variability of economically important traits in all studied cultivars depended on heat and moisture availability. In the conditions of the western part of the Central region, the level of variability of the main economically important traits was higher, namely, the variation coefficient reached 16.2% for plant height, 13.9% for technical length, and 27.0% for stem diameter. In the Middle Volga region, the maximum variability values were noted at 13.6% for plant height, 13.4% for technical length, and 19.8% for stem diameter. Economically important traits characterizing the generative structure are more variable than vegetative ones: the maximum level of variability was recorded at 46% for seed weight per plant in the Penza Region and 63.9% in the Smolensk Region. Using mathematical analysis methods, it was established that among the studied cultivars ‘Vera’ exhibited high plasticity: the linear regression coefficient for environmental conditions according to Eberhart and Russell (bi) was 1.4, and low stability: the weighted average of absolute scores (WAASВ) was 54.4; and the yield and stability balance index (WAASBY) was 50.0. Cv. ‘Surskaya’ demonstrated high stability with WAASВ=19.7, and WAASBY=52.7, and cv. ‘Vera’ demonstrated the highest seed yield in both test zones.

About the Authors

I. V. Ushchapovsky
Federal Scientific Center of Bast Crops
Россия

Igor V. Ushchapovsky, Cand. Sci. (Biology), Deputy Director for Research, FRCBGC 

17/56, Komsomolsky Avenue, Tver, 170041 Russia



N. S. Shimanskaya
Federal Scientific Center of Bast Crops
Россия

Natalya S. Shimanskaya, Cand. Sci. (Agriculture), Leading Researcher, FRCBGC

17/56, Komsomolsky Avenue, Tver, 170041 Russia



V. A. Serkov
Federal Scientific Center of Bast Crops
Россия

Valerian A. Serkov, Dr. Sci. (Agriculture), Chief Researcher, FRCBGC

1 B, Michurina Street, Lunino Workers' Settlement, Penza Region, 442731 Russia



S. V. Ivanova
Federal Scientific Center of Bast Crops
Россия

Svetlana V. Ivanova, Junior Researcher, FRCBGC 

21, Nakhimov Street, Smolensk, 214025 Russia



References

1. Abdollahi M., Sefidkon F., Calagari M., Mousavi A., Mahomoodally M.F. A comparative study of seed yield and oil composition of four cultivars of hemp (Cannabis sativa L.) grown from three regions in northern Iran. Industrial Crops and Products. 2020;152:112397. DOI: 10.1016/j.indcrop.2020.112397

2. Alsaleh A., Yılmaz G. Exploring cannabidiol variations, investigation of genetic diversity, population structure and unveiling male-specific genetic marker in industrial hemp (Cannabis sativa L.). Genetic Resources and Crop Evolution. 2025;72:797-814. DOI: 10.1007/s10722-024-02015-1

3. Amarasinghe P., Pierre C., Moussavi M., Geremew А., Woldesenbet S., Weerasooriya A. The morphological and anatomical variability of the stems of an industrial hemp collection and the properties of its fibres. Heliyon. 2022;8(4):e09276. DOI: 10.1016/j.heliyon.2022.e09276

4. Anwar F., Latif S., Ashraf M. Analytical characterization of hemp (Cannabis sativa) seed oil from different agro-ecological zones of Pakistan. Journal of the American Oil Chemists' Society. 2006;83:323-329. DOI: 10.1007/s11746-006-1207-x

5. Babaei M., Nemati H., Arouiee H., Torkamaneh D. Characterization of indigenous populations of cannabis in Iran: a morphological and phenological study. BMC Plant Biology. 2024;24(151):1-21. DOI: 10.1186/s12870-024-04841-y

6. Bedak G.R. (comp.). Guidelines for conducting field and vegetative experiments with hemp (Metodicheskie ukazaniia po provedeniiu polevykh i vegetatsionnykh opytov s konoplyoi). Moscow: VASKHNIL; 1980. [in Russian]

7. Chaisan T., Thobunluepop P., Thongthip N., Rakpenthai А., Puangsin В., Samipak S., Pluempanupat W. Identification of morphological traits affecting high seed yield potential from new hemp germplasm collected in Thailand. Chilean journal of agricultural research. 2025;85(1):88-97. DOI: 10.4067/S0718-58392025000100088

8. CRAN. The R Project for Statistical Computing. Available from: https://www.r-project.org/ [accessed Sept. 17, 2025].

9. Dospekhov B.A. Methodology of field trial with fundamentals of statistical processing of research results (Metodika polevogo opyta s osnovami statisticheskoy obrabotki rezultatov issledovaniy). Moscow; 2012. [in Russian]

10. Eberhart S.A., Russell W.A. Stability parameters for comparing varieties. Crop Science. 1966;6(1):36-40. DOI: 10.2135/cropsci1966.0011183X000600010011x

11. Egorova N.N., Kardashevskaya V.E. The structure of the Variability of Morphological Features Agrostis diluta Kurcz. Vestnik of M.K. Ammosov North-Eastern Federal University. 2016;6(56):5-15. [in Russian]

12. Hammami N., Privé J.-P., Moreau G. Spatiotemporal variability and sensitivity of industrial hemp cultivars under variable field conditions. European Journal of Agronomy. 2022;138:126549. DOI: 10.1016/j.eja.2022.126549

13. Krylova E.A., Chunikhina O.A., Boyko A.P., Miroshnichenko E.V., Khlestkina E.K., Burlyaeva M.O. Variability of morphological and phenological traits in Vigna unguiculata (L.) Walp. accessions contrasting by growth type in different ecological and geographical conditions. Plant Biotechnology and Breeding. 2024;7(2):16-30. [in Russian]. DOI: 10.30901/2658-6266-2024-2-o7

14. Mamaev S.A. Basic principles of the methodology for studying intraspecific variability of woody plants (Osnovnye printsipy metodiki issledovaniia vnutrividovoi izmenchivosti drevesnykh rastenii). In: Individual and eco-geographical variability of plants = Individualnaia i ekologo-geograficheskaia izmenchivost rastenii. Sverdlovsk: Ural Scientific Center of the USSR Academy of Sciences; 1975. p.3-14. [in Russian]

15. Mostafaei Dehnavi M., Damerum A., Taheri S., Ebadi A., Panahi S., Hodgin G., Brandley B., Salami S.A., Taylor G. Population genomics of a natural Cannabis sativa L. collection from Iran identifies novel genetic loci for flowering time, morphology, sex and chemotyping. BMC Plant Biology. 2025;25(1):80. DOI: 10.1186/s12870-025-06045-4

16. Olivoto T. Analyzing multienvironment trials using BLUP. 2023. Available from: https://tiagoolivoto.github.io/metan/articles/vignettes_blup.html [accessed Nov. 17, 2025].

17. Olivoto T., Lúcio A.D. metan: An R package for multi-environment trial analysis. Methods in Ecology and Evolution. 2020;11(6):783-789. DOI: 10.1111/2041-210X.13384

18. Olivoto T., Lúcio A.D.C., Silva J.A.G., Sari B.G., Diel M.I. Mean performance and stability in multi-environment trials II: Selection based on multiple traits. Agronomy Journal. 2019;111(6):2961-2969. DOI: 10.2134/agronj2019.03.0221

19. Pavlovic R., Panseri S., Giupponi L., Leoni V., Citti C., Cattaneo C., Cavaletto M., Giorgi A. Phytochemical and ecological analysis of two varieties of hemp (Cannabis sativa L.) grown in a mountain environment of Italian Alps. Frontiers in Plant Science. 2019;10:1265. DOI: 10.3389/fpls.2019.01265

20. Petit J., Salentijn E.M.J., Paulo M.-J., Thouminot C., van Dinter B.J., Magagnini G., Gusovius H.-J., Tang K., Amaducci S., Wang S., Uhrlaub B., Müssig J., Trindade L.M. Genetic variability of morphological, flowering, and biomass quality traits in hemp (Cannabis sativa L.). Frontiers in Plant Science. 2020;11:102. DOI: 10.3389/fpls.2020.00102

21. Pustovoit V.S. Selected Works (Izbrannye trudy). Moscow: Kolos; 1966. [in Russian]

22. Selyaninov G.T. On agricultural climate assessment. (O sel'skokhozyaystvennoy otsenke klimata). Works on agricultural meteorology = Trudy po sel'skokhozyaystvennoy meteorologii. 1928;20:165-177. [in Russian]

23. Serkov V.A Formation of a new initial material for the development of innovative directions of breeding hemp. International agricultural journal. 2022;(5)(389):517-520. [in Russian]. DOI: 10.55186/25876740_2022_65_5_517

24. Serkov V.A., Koshelyaev V.V., Davydova O.K. Characteristics of the main morphological features of the source material of non-narcotic monoecious hemp. Niva Povolzhya. 2024;(2)(70):1008. [in Russian]. DOI: 10.36461/NP.2024.70.2.013

25. Serkov V.A., Zelenina O.N., Smirnov A.A., Pluzhnikova I.I., Salnikov S.V., Zelenin I.N. Cultivation of Central Russian monoecious hemp in the forest-steppe of the Middle Volga region: (Practical recommendations). Penza; 2011. [in Russian]. URL: https://www.cnshb.ru/Vexhib/volk/12_4754.pdf [дата обращения: 01.11.2025]

26. Shimanskaia N.S., Ivanova S.V., Bakulova I.V., Serkov V.A. Features of cultivation of hemp of the Central Russian ecotype in the conditions of the Western part of the Central region (Osobennosti vozdelyvaniia konopli posevnoi Srednerusskogo ekotipa v usloviyakh Zapadnoi chasti Tsentral’nogo regiona). In: Current Issues in the Biology, Breeding, and Agricultural Engineering of Garden Crops: a collection of papers from the International Scientific and Practical Conference dedicated to the 100th anniversary of the birth of Academician G.I. Tarakanov (Aktual'nyye voprosy biologii, selektsii i agrotekhniki sadovykh kul'tur: sbornik trudov Mezhdunarodnoy nauchno-prakticheskoy konferentsii, posvyashchennoy 100-letiyu so dnya rozhdeniya akademika G.I. Tarakanova); 2023 October 31; Moscow, Russia. Moscow: RGAU-MSKHA im. K.A. Timiriazeva; 2023. p.73-76. [in Russian]. URL: https://www.elibrary.ru/download/elibrary_65599700_32487313.pdf [дата обращения: 17.09.2025]

27. Shimanskaya N.S., Ivanova S.V., Serkov V.A., Uschapovsky I.V. Influence of agroecological conditions on the formation of economically valuable traits of hemp. In: Genetic and Radiation Technologies in Agriculture: Proceedings of the 3rd International Young Scientists Conference; 2024 October 23–24; Obninsk, Russia. Obninsk: NRC «Kurchatov Institute» – RIRAE; 2024. p.292-295. [in Russian]. URL: https://elibrary.ru/item.asp?id=81280501 [дата обращения: 17.09.2025]

28. Sraka M., Škevin D., Obranović M., Butorac J., Magdić I. Agroecological conditions of industrial hemp production in the western Pannonian agricultural subregion and fatty acids composition of hemp seed oil. Journal of Central European Agriculture. 2019;20(3):809-822. [in Croatian]. DOI: 10.5513/JCEA01/20.3.2529

29. State Register = GOST R 54650-2011. Soils. Determination of mobile phosphorus and potassium compounds using the Kirsanov method as modified by the Central Institute of Agrochemistry (CINAO). Moscow: Standartinform; 2019. [in Russian]

30. State Register = GOST 26213-2021. Soils. Methods for determining organic matter. Moscow: Russian Institute of Standardization; 2021. [in Russian]

31. Trubanová N., Isobe S., Shirasawa K., Watanabe A. Genome-specific association study (GSAS) for exploration of variability in hemp (Cannabis sativa). Scientific Reports. 2025;15(1):8371. DOI: 10.1038/s41598-025-92168-5

32. Trubanová N., Pender G., McCabe P.F., Melzer R., Schilling S. Exploring phenotypic and genetic variability in hemp (Cannabis sativa). bioRxiv. The Preprint Server for Biology. 2023. DOI: 10.1101/2023.11.01.565084

33. Tsaliki E., Kalivas A., Jankauskiene Z., Irakli M., Cook C., Grigoriadis I., Panoras I., Vasilakoglou I., Dhima K. Fibre and seed productivity of industrial hemp (Cannabis sativa L.) varieties under Mediterranean conditions. Agronomy. 2021;11(1):171. DOI: 10.3390/agronomy11010171

34. Uschapovsky I.V., Novikov E.V., Basova N.V., Bezbabchenko A.V., Galkin A.V. System problems of flax growing in Russia and abroad, the possibilities of their solution. Dairy Herald. 2017;1(25):166-186. [in Russian]

35. Vasilkova T.M., Makovetsky V.V., Maksimov M.M. Handbook of economics and management in the agro-industrial complex. Moscow: ICC Kolos-s; 2022. [in Russian]

36. Vavilov N.I. Selected Works (Izbrannye sochineniyia). Moscow: Kolos; 1966. [in Russian]

37. Vonapartis E., Aubin M.P., Seguin P., Mustafa A.F., Charron J.B. Seed composition of ten industrial hemp cultivars approved for production in Canada. Journal of Food Composition and Analysis. 2015;39:8-12. DOI: 10.1016/j.jfca.2014.11.004

38. Younas M., Qureshi R., van Velzen R., Mashwani Z.U.R., Saqib Z., Ali A., Rehman S., Farah M.A., Al-Anazi K.M. Geo-climatic factors co-drive the phenotypic diversity of wild hemp (Cannabis sativa L.) in the Potohar Plateau and Lesser Himalayas. BMC Plant Biology. 2024;24(1031):1-15. DOI: 10.1186/s12870-024-05730-0

39. Zykin V.A., Belan I.A., Yusov V.S., Nedorezkov V.D., Ismagilov R.R., Kadikov R.K., Islamgulov D.R. Methodology for calculating and assessing the parameters of ecological plasticity of agricultural plants. Ufa; 2015. [in Russian]


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For citations:


Ushchapovsky I.V., Shimanskaya N.S., Serkov V.A., Ivanova S.V. Variability of economically important traits of hemp depending on the region of cultivation. Plant Biotechnology and Breeding. (In Russ.) https://doi.org/10.30901/2658-6266-2025-4-o8

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